با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری رشته بیوتکنولوژی کشاورزی، دانشگاه آزاد اسلامی واحد سبزوار، سبزوار، ایران.

2 استادیار، دانشگاه حکیم سبزواری، سبزوار، ایران.

3 دانشیار، دانشگاه حکیم سبزواری، سبزوار، ایران.

4 دانشیار، دانشگاه آزاد اسلامی سبزوار، سبزوار، ایران.

چکیده

تنش‌های غیر زیستی و کالوس‌زایی می‌توانند سبب افزایش ترکیبات فنلی شوند. هدف از این پژوهش افزایش مقدار فنل‌کل و فعالیت آنتی‌اکسیدانی در گیاه دارویی عدس‌الملک (Securigera securidaca) و کالوس‌های حاصل از آن به‌وسیله تنش فراصوت، در شرایط درون آزمایشگاهی بود. گیاهان در گلخانه رشد کردند. کالوس‌زایی با محیط کشت MS حاوی 2/2 میلی‌گرم بر لیتر آلفا نفتالیک استیک اسید و 8/8 میلی‌گرم از 6-بنزیل آمینو پورین انجام شد. تنش فراصوت بر روی گیاه اصلی به‌مدت 10، 20 و 30 دقیقه و در طی 15 روز، در شرایط درون آزمایشگاهی اعمال شد. تنش فراصوت بر روی کالوس به‌مدت 10 دقیقه و به‌مدت 15 روز صورت گرفت. نمونه‌ها در روزهای گوناگون (روز اول، پنجم، دهم و پانزدهم) جمع‌آوری شدند. سپس نمونه‌ها در آون خشک و عصاره اتانولی از آن‌ها تهیه شد. آزمون‌های تعیین مقدار فنل‌کل و فعالیت آنتی‌اکسیدانی به‌وسیله روش‌های Folin-Ciocalteu و Wu بر روی برگ‌های گیاه اصلی و کالوس آن انجام شد. نتایج نشان دادند بیشترین مقدار فنل کل در گیاه در تنش فراصوت، در روز اول و تیمار 30 دقیقه بود. در تمامی تیمارها، فعالیت آنتی‌اکسیدانی نسبت به نمونه شاهد کاهش یافته بود. بیشترین مقدار فنل‌کل در کالوس‌های در تیمار تنش فراصوت، در روز پانزدهم مشاهده شد. فعالیت آنتی‌اکسیدانی در کالوس‌های در تنش فراصوت نسبت به نمونه کنترل کاهش یافت؛ بنابراین می‌توان نتیجه گرفت که تنش فراصوت در نمونه‌های این آزمایش سبب افزایش مقدار فنل‌کل شد اما بر فعالیت آنتی‌اکسیدانی تأثیری نداشت. همچنین کالوس‌زایی و تنش فراصوت به‌طور هم‌زمان سبب افزایش مقدار فنل‌کل می‌گردد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of ultrasonic stress on the amount of total phenolic content and antioxidant activity in the Securigera Securidaca L. leaves and callus

نویسندگان [English]

  • Mona Faraji Heriss 1
  • Mohammad Reza Vaezi Kakhki 2
  • Nasrin Mollania 3
  • Mohammad Armin 4

1 Ph.D Student, Department of Agricultural Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

2 Assistant Professor of Plant Physiology and Genetics, Hakim Sabzevari University, Sabzevar, Iran.

3 Associate Professor of Biochemistry, Hakim Sabzevari University, Sabzevar, Iran.

4 Associate Professor, Department of Agronomy, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

چکیده [English]

Abiotic stress and callus formation can increase total phenolic content and antioxidant activity. The aim of this study was to increase the amount of total phenol and antioxidant activity in Securigera securidaca L. and its calli by ultrasonic stress in vitro conditions. The plants were grown in the greenhouse. Callus formation was performed with MS culture medium containing α-naphthalene acetic acid (2.2 mg/L) and 6-Benzylaminopurine (8.8 mg/L). Ultrasonic stress was applied to the main plants for 10, 20 and 30 minutes in 15 days in vitro conditions; in addition, the ultrasonic stress was applied to the calli for 10 minutes at the same time. The samples were collected on the first, fifth, tenth and fifteenth days. The samples were then dried in an oven, then their ethanolic extract was prepared. Total phenol content and antioxidant activity tests were performed by Folin-Ciocalteu and Wu methods respectively on the leaves of the main plant and its calli. The results showed that the amount of total phenol in the 30 minutes on the first day of ultrasonic treatment is higher than the control. In all treatments, antioxidant activity was reduced compared to the control sample. The highest amount of phenol was observed in calli in ultrasonic stress treatment on the 15th day. Antioxidant activity was reduced in calli under ultrasonic stress compared to the control sample. Therefore, it can be concluded that ultrasonic stress in the samples of this experiment increased the amount of phenolic but did not affect the antioxidant activity. Callus formation and ultrasonic stress also increase total phenol content simultaneously.

کلیدواژه‌ها [English]

  • abiotic stress
  • medicine plants
  • secondary metabolism
  • ultrasonic stress
Aboulghazi, A., Bakour, M., Fadil, M., & Lyoussi, B. (2022). Simultaneous Optimization of Extraction Yield, Phenolic Compounds and Antioxidant Activity of Moroccan Propolis Extracts: Improvement of Ultrasound-Assisted Technique Using Response Surface Methodology. Processes,10(2),297-313. Afkhami, F., Zare, N., Asghari-Zakaria, R., & Mehdizadeh, M. (2020). The Effects of Ultrasound, Temperature, Light, Chitosan and Plant Growth Regulators on Callus Induction in Saffron (Crocus sativus L.). Saffron Agronomy & Technology, 8(3), 361-375. (in Persian) Afzal, J., Hu, C., Imtiaz, M., Elyamine, A., Rana, M., Imran, M., & Farag, M. (2019). Cadmium tolerance in rice cultivars associated with antioxidant enzymes activities and Fe/Zn concentrations. International Journal of Environmental Science and Technology, 16(8), 4241-4252. Ampofo, J. O., & Ngadi, M. (2020). Ultrasonic assisted phenolic elicitation and antioxidant potential of common bean (Phaseolus vulgaris) sprouts. Ultrasonics Sonochemistry,64, 974-104. Brand-Williams, W., Cuvelier, M.-E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30. da Rocha, T. S., de Lima, A., Silva, J. d. N., Sampaio, G.R., Soares Freitas, R. A. M., Danielski, R.,.. . Torres, E.A.F.d.S. (2022). Vitamin C and Phenolic Antioxidants of Jua (Ziziphus joazeiro M.) Pulp: A Rich Underexplored Brazilian Source of Ellagic Acid Recovered by Aqueous Ultrasound-Assisted Extraction. Molecules, 27(3), 627-637 da Silva, J. A. T., & Dobránszki, J. (2014). Sonication and ultrasound: impact on plant growth and development. Plant Cell, Tissue and Organ Culture (PCTOC), 117(2), 131-143. da Silva, J. A. T., Hidvégi, N., Gulyás, A., Tóth, B., & Dobránszki, J. (2020). Transcriptomic response of in vitro potato (Solanum tuberosum L.) to piezoelectric ultrasound. Plant Molecular Biology Reporter, 38(3), 404-418. Ding, J., Hou, G. G., Dong, M., Xiong, S., Zhao, S., & Feng, H. (2018). Physicochemical properties of germinated dehulled rice flour and energy requirement in germination as affected by ultrasound treatment. Ultrasonics Sonochemistry,41, 484-491. Ding, J., Hou, G. G., Nemzer, B. V., Xiong, S., Dubat, A., & Feng, H. (2018). Effects of controlled germination on selected physicochemical and functional properties of whole-wheat flour and enhanced γ-aminobutyric acid accumulation by ultrasonication. Food Chemistry, 243, 214-221. Dobránszki, J., Asbóth, G., Homoki, D., Bíró-Molnár, P., da Silva, J. A. T., & Remenyik, J. (2017). Ultrasonication of in vitro potato single node explants: activation and recovery of antioxidant defence system and growth responses. Plant Physiology and Biochemistry, 121, 153-160. Gani, A., Baba, W. N., Ahmad, M., Shah, U., Khan, A. A., Wani, I. A.,.. . Gani, A. (2016). Effect of ultrasound treatment on physico-chemical, nutraceutical and microbial quality of strawberry. LWT-Food Science and Technology, 66, 496-502. Ghori, N.-H., Ghori, T., Hayat, M., Imadi, S., Gul, A., Altay, V., & Ozturk, M. (2019). Heavy metal stress and responses in plants. International journal of Environmental Science and Technology, 16(3), 1807-1828. Gratão, P. L., Monteiro, C. C., Tezotto, T., Carvalho, R. F., Alves, L. R., Peters, L. P., & Azevedo, R. A. (2015). Cadmium stress antioxidant responses and root-to-shoot communication in grafted tomato plants. BioMetals, 28(5), 803-816. Hassanien, R. H., Hou, T. Z., Li, Y. F., & Li, B. M. (2014). Advances in effects of sound waves on plants. Journal of Integrative Agriculture, 13(2), 335-348. Isbilir, S. S., & Sagiroglu, A. (2013). Total phenolic content, antiradical and antioxidant activities of wild and cultivated Rumex acetosella L. extracts. Biological agriculture & horticulture, 29(4), 219-226. Jamshidzadeh, A., Pasdaran, A., & Heidari, R. (2018). Pharmacognostic and anti-inflammatory properties of Securigera securidaca seeds and seed oil. Research Journal of Pharmacognosy, 5(3), 31-39. (in Persian) Kabera, J. N., Semana, E., Mussa, A. R., & He, X. (2014). Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. J Pharm Pharmacol, 2, 377-392. Karakas, F. P., Sahin, G., Turker, A. U., & Verma, S. K. (2022). Impacts of heavy metal, high temperature, and UV radiation exposures on Bellis perennis L. (common daisy): Comparison of phenolic constituents and antioxidant potential (enzymatic and non-enzymatic). South African Journal of Botany, 147, 370-379. Kartal, M., Konuklugil, B., Indrayanto, G., & Alfermann, A. (2004). Comparison of different extraction methods for the determination of podophyllotoxin and 6-methoxypodophyllotoxin in Linum species. Journal of pharmaceutical and biomedical analysis, 35(3), 441-447. Liu, Y., Yoshikoshi, A., Wang, B., & Sakanishi, A. (2003). Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells. Colloids and Surfaces B: Biointerfaces,27(4),287-293. Lu, C., Ding, J., Park, H. K., & Feng, H. (2020). High intensity ultrasound as a physical elicitor affects secondary metabolites and antioxidant capacity of tomato fruits. Food Control, 113, 107176. Mishra, R. C., Ghosh, R., & Bae, H. (2016). Plant acoustics: in the search of a sound mechanism for sound signaling in plants. Journal of experimental botany, 67(15), 4483-4494. Namdeo, A. (2007). Plant cell elicitation for production of secondary metabolites: a review. Pharmacogn Rev, 1(1), 69-79. Nicolai, M., Pereira, P., Vitor, R. F., Reis, C. P., Roberto, A., & Rijo, P. (2016). Antioxidant activity and rosmarinic acid content of ultrasound-assisted ethanolic extracts of medicinal plants. Measurement, 89, 328-332. Rao, P. R., & Rathod, V. K. (2015). Mapping study of an ultrasonic bath for the extraction of andrographolide from Andrographis paniculata using ultrasound. Industrial Crops and Products, 66, 312-318. Rezaei, A., Ghanati, F., Behmanesh, M., & Mokhtari-Dizaji, M. (2011). Ultrasound-potentiated salicylic acid–induced physiological effects and production of taxol in hazelnut (Corylus avellana L.) cell culture. Ultrasound in medicine & biology, 37(11), 1938-1947. Roberts, S. C., & Shuler, M. L. (1997). Large-scale plant cell culture. Current opinion in Biotechnology, 8(2),154-159. Rokhina, E. V., Lens, P., & Virkutyte, J. (2009). Low-frequency ultrasound in biotechnology: state of the art. Trends in biotechnology, 27(5), 298-306. Rudolf, J. R., & Resurreccion, A. V. (2005). Elicitation of resveratrol in peanut kernels by application of abiotic stresses. Journal of Agricultural and Food Chemistry, 53(26), 10186-10192. Sales, J. M., & Resurreccion, A. V. A. (2010). Phenolic profile, antioxidants, and sensory acceptance of bioactive-enhanced peanuts using ultrasound and UV. Food Chemistry, 122(3), 795-803. Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299, 152-178. Tofighi, Z., Sabzevari, O., Rezaei Taleqani, Z., & Yassa, N. (2016). Investigation of securigera securidaca seeds extract and different fractions on serum glucose, blood factors and liver morphology in diabetic animals. Iranian Journal of Endocrinology and Metabolism, 18(1), 37-45. (in Persian) Wang, B., Zhou, J., Wang, Y., Zhu, L., & Teixeira da Silva, J. (2006). Physical stress and plant growth. Floriculture, ornamental and plant biotechnology: advances and topical issues, 2, 68-85. Wrona, M., Blasco, S., Becerril, R., Nerin, C., Sales, E., & Asensio, E. (2019). Antioxidant and antimicrobial markers by UPLC®–ESI-Q-TOF-MSE of a new multilayer active packaging based on Arctostaphylos uva-ursi. Talanta, 196, 498-509. Wu, J., & Lin, L. (2002). Ultrasound‐Induced Stress Responses of Panax ginsengCells: Enzymatic Browning and Phenolics Production. Biotechnology Progress, 18(4), 862-866. Wu, J., & Lin, L. (2003). Enhancement of taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction. Applied microbiology and biotechnology, 62(2), 151-155. Yeoh, W. K., & Ali, A. (2017). Ultrasound treatment on phenolic metabolism and antioxidant capacity of fresh-cut pineapple during cold storage. Food Chemistry, 216, 247-253. Yu, J., Engeseth, N. J., & Feng, H. (2016). High Intensity Ultrasound as an Abiotic Elicitor-Effects on Antioxidant Capacity and Overall Quality of Romaine Lettuce. Food and Bioprocess Technology, 9(2), 262-273. Yue, W., Ming, Q.-l., Lin, B., Rahman, K., Zheng, C.-J., Han, T., & Qin, L.-p. (2016). Medicinal plant cell suspension cultures: pharmaceutical applications and high-yielding strategies for the desired secondary metabolites. Critical reviews in biotechnology, 36(2), 215-232. Zannou, O., Pashazadeh, H., Ibrahim, S. A., Koca, I., & Galanakis, C. M. (2022). Green and Highly Extraction of Phenolic Compounds and Antioxidant Capacity from Kinkeliba (Combretum micranthum G. Don) by Natural Deep Eutectic Solvents (NADESs) using Maceration, Ultrasound-assisted Extraction and Homogenate-assisted Extraction. Arabian Journal of Chemistry, 103752. Zanon, M., Davis, B. A., Marquer, L., Brewer, S., & Kaplan, J. O. (2018). European forest cover during the past 12,000 years: a palynological reconstruction based on modern analogs and remote sensing. Frontiers in plant science, 9, 253.