بررسی عملکرد پروموتر AtPHT1;1 در سیستم هترولوگ کلزا

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی مقطع دکتری تخصصی، پژوهشگاه ملی مهندسی ژنتیک و زیست‌فناوری، شهرک علم و فناوری پژوهش، بلوار پژوهش، تهران، ایران.

2 استادیار، گروه پژوهشی مهندسی ژنتیک و ایمنی زیستی، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران.

3 دانش‌آموخته دکتری تخصصی، مؤسسه تحقیقاتی ژنتیک گیاهی و گیاهان زراعی لاییبنیز، IPK، آلمان.

4 استاد، پژوهشگاه ملی مهندسی ژنتیک و زیست‌فناوری، شهرک علم و فناوری پژوهش، بلوار پژوهش، تهران، ایران.

چکیده

اصلاح گیاهان زراعی با قابلیت بیشتر استفاده از مواد معدنی موجود در خاک از اهداف پژوهشگران حوزه زیست‌فناوری است. روش‌های مهندسی ژنتیک پیشرفت‌های چشمگیری را در اصلاح گیاهان زراعی با انتقال و ایجاد صفات مفید جهت تولید بیشتر در شرایط عادی و یا تحت تنش را فراهم می‌آورند. طراحی سازه‌های ژن کارآمد دارای پروموترهایی با عملکرد مناسب به‌منظور بیان اختصاصی ژن‌های مورد نظر در بافت‌های هدف و در زمان مناسب جهت ایجاد صفات مطلوب مانند تحمل به تنش‌های زیستی و غیر زیستی یا اهداف دیگر از اهمیت ویژه‌ای برخوردار است. بیان اختصاصی ژن‌های ناقل فسفات در ریشه و القای آنها با تنش کمبود فسفات، قابلیت پروموترهای اعضای این خانواده ژن را جهت بهره‌برداری در گیاهان تراریخت به‌ویژه برای جذب فسفات از خاک نشان‌می‌دهد. در این پژوهش یک قطعه 1826 جفت بازی مربوط به پروموتر ژن AtPHT1;1 گیاه آرابیدوپسیس تالیانا (Arabidopsis thaliana L.) مورد بررسی بیوانفورماتیکی قرارگرفت و نتایج نشان داد که این پروموتر دارای موتیف‌های متعدد مربوط به‌بیان اختصاصی در ریشه است. بیان اسید فسفات از ترشحی AtPAP17 به‌عنوان یک ژن گزارشگر تحت کنترل پروموتر AtPHT1;1 در گیاهان تراریخت کلزا نشان داد که پروموتر مذکور ویژگی بیان بافتی خود را در گیاه کلزا حفظ کرده و به‌طور اختصاصی در ریشه بیان می‌شود و می‌تواند به‌عنوان یک ناحیه تنظیم‌کننده برای بیان اختصاصی ژن‌های مطلوب در ریشه گیاهان تراریخت کلزا مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Functional Analysis of AtPHT1;1 promoter in rapeseed (Brassica napus L.) heterologous system

نویسندگان [English]

  • Zahra Fathi 1
  • Katayoun Zamani 2
  • Solmaz Khosravi 3
  • Mohammad Malboobi 4
1 Ph.D. Student, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Tehran, I.R. Iran.
2 Assistant Professor, Department of Genetic Engineering and Biosafety, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, Iran.
3 Ph.D, Leibniz Institute of Plant Genetics and Crop Plant Research, IPK Gatersleben, Germany.
4 Professor, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Tehran, I.R. Iran.
چکیده [English]

Breeding crops with a higher ability in using soil minerals is one of the biotechnology researchers’ goals. Genetic engineering methods provide considerable advances in crop breeding by transferring and creating desired traits for further production under normal or stress conditions. In these procedures, Design of efficient gene constructs is of particular importance and requires promoters with proper function to specifically express the gene of interest in the target tissue and at the appropriate time to develop desired traits such as tolerance to biotic and abiotic stresses or other aims. Specific expression of phosphate-transporter genes in the roots and their induced levels in phosphate deficiency shows the potential of this gene-family promoters utilization in transgenic plants, particularly for the use in phosphate absorption from soil. Bioinformatics analysis showed that the 1826-bp promoter fragment of AtPHT1;1 gene carries several motifs leading to root-specific expression in Arabidopsis thaliana. The expression of a secretory acid phosphatase gene, AtPAP17, as a reporter gene in rapeseed transgenic plants indicated that the AtPHT1;1 promoter retains its root-specific criteria in rapeseed such that it could be used as a regulatory region for the specific expression of desired genes in transgenic rapeseed plant roots.

کلیدواژه‌ها [English]

  • ATPHT1 1"
  • promoter "
  • purple"
  • acid "
  • "
  • phosphatase"
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