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تجزیه و تحلیل جامع خانواده عامل رونویسی ERF و بیان آن‌ها در کنجد تحت تنش‌های غیرزنده

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه بیوتکنولوژی و اصلاح‌نباتات، دانشگاه کشاورزی و منابع طبیعی ساری، ساری، ایران.

2 دانشیار، گروه بیوتکنولوژی و اصلاح‌نباتات، دانشگاه کشاورزی و منابع طبیعی ساری، ساری، ایران.

3 استادیار، پژوهشکده ژنتیک و زیست‌فناوری کشاورزی طبرستان، دانشگاه کشاورزی و منابع طبیعی ساری، ساری، ایران.

4 استادیار، گروه علوم پایه، دانشگاه کشاورزی و منابع طبیعی ساری، ساری، ایران.

چکیده

کنجد (Sesamum indicum L.) یک گیاه زراعی دانه روغنی مهم از نظر تغذیه‌ای و دارویی می‌باشد که تنش‌های محیطی ظرفیت عملکرد آن را محدود می‌کند. عامل پاسخ دهنده به اتیلن (ERF) یکی از بزرگترین خانواده‌های عوامل رونویسی می‌باشد که در تنظیم پاسخ گیاه به تنش‌های غیر زنده نقش کلیدی ایفا می‌کند. در مطالعه حاضر، در مجموع 113 ژن ERF از ژنوم کنجد شناسایی شد، که آن‌ها خود به دو زیرخانواده شامل 46 عضو متصل به عناصر پاسخ دهنده به پسابیدگی (DREB) و 67 عضو ERF تقسیم شدند. روابط فیلوژنتیکی، خصوصیات فیزیکوشیمیایی پروتئین‌ها، ساختارهای ژنی و موتیف‌های آمینو اسیدی حفاظت شده در خانواده ERF کنجد مورد تجزیه و تحلیل قرار گرفت. در ادامه پروفایل بیانی ژن‌های ERF کنجد در بافت‌های مختلف و همچنین تحت تنش‌های محیطی بررسی گردید. به‌طور کلی ژن‌های متعدد از خانواده ERF در بافت‌های مختلف کنجد به‌ویژه در ریشه، کپسول و گل از بیان قابل ملاحظه‌ای برخوردار بودند. همچنین پروفایل‌های بیانی نشان داد ژن‌های RAP2.2L، PTI6، ERF017L و ERF096 به‌ترتیب تحت تنش‌های خشکی، اسمزی، شوری و غرقاب بشدت القا شدند. افزون بر این، نتایج qPCR نشان داد که بیان نسبی ژن ERF061L در ژنوتیپ متحمل کنجد در مقایسه با حساس تحت شرایط خشکی بیشتر می‌باشد. این مطالعه داده‌های مهمی را برای درک تکامل و عملکرد خانواده ERF در کنجد فراهم نموده است که می‌تواند در برنامه‌های اصلاحی آینده برای تحمل تنش‌های غیر زنده مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Comprehensive analysis of ERF transcription factor family and their expression in sesame under abiotic stresses

نویسندگان [English]

  • Mohammad Amin Baghery 1
  • Seyed Kamal Kazemitabar 2
  • Ali Dehestani 3
  • Pooyan Mehrabanjoubani 4
  • Hamid Najafi Zarrini 2

1 Ph.D. Candidate, Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

2 Associate Professor, Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

3 Assistant Professor, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

4 Assistant Professor, Department of Basic Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

چکیده [English]

Sesame (Sesamum indicum L.) is a nutritionally and medicinally important oilseed crop that environmental stresses limit its yield potential. Ethylene-responsive factor (ERF) is one of the largest transcription factor families that play key roles in regulating plant response to abiotic stress. In the current study, a total of 113 ERF genes were identified from the sesame genome and they were divided into two subfamilies including, 46 dehydration-responsive element-binding (DREB) members, and 67 ERF members. Phylogenetic relationships, physicochemical properties of proteins, structural properties of genes, and conserved amino acid motifs in the sesame ERF family were analyzed. Then, the expression profile of sesame ERF genes in various tissues as well as under environmental stresses was investigated. Overall, several genes of the ERF Family were expressed noticeably in different sesame tissues, especially in roots, capsules, and flowers. Expression profiles also showed that RAP2.2L, PTI6, ERF017L, and ERF096 genes were strongly induced by drought, osmotic, salinity, and waterlogging stresses, respectively. Moreover, the qPCR results showed that the relative expression of the ERF061L gene was higher in the sesame tolerant genotype compared to the susceptible one under drought conditions. This study provides important data for understanding the evolution and functions of the ERF family in sesame that can be used in future breeding programs for abiotic stresses tolerance.

کلیدواژه‌ها [English]

  • Environmental stress
  • ERF Transcription factors
  • Gene expression
  • Phylogenetic relationships
  • Sesame
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فصلنامه علمی زیست فناوری گیاهان زراعی
دوره 11، شماره 35 - شماره پیاپی 35
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صفحه 1-21
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